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Encompassing Amino Acids: 224-529
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP.
Background: Mammalian PTPases can be subdivided into 1 of 2 broad categories: transmembrane receptor PTPases and intracellular PTPases. PTPN11 is one of the 2 closely related mammalian intracellular PTPases whose sequences encode 2 tandem SRC homology 2 (SH2) domains that are located at the amino-terminal side of a single PTPase catalytic domain. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.
Description: Protein tyrosine phosphatase SHP-2, also known as PTPN11 (GenBank accession number NM_002834), a.a. 224-529 (catalytic domain) with N-terminal GST-tag, MW=62 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
Genbank: NM_002834
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: Q06124
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. B.G. Neel et al. Trends Biochem. Sci. (2003) 28:284.
2. J.V. Ravetch and L.L. Lanier Science (2000) 290:84.
3. S. Yamazaki et al. J. Infect. Dis. (2003) 187:334.