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Encompassing Amino Acids: 102-399
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM HDAC substrate 1 (Catalog #50032), 500 µM NAD+ and Sirtuin 3 (31 - 2000 ng). Incubation condition: 30 min at 37°C followed bytreatment with 50 µl of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
Description: Human Sirtuin 3 (GenBank Accession No. NM_012239), a.a. 102-399 with N-terminal GST-tag, MW= 59 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_012239
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9NTG7
Unit Definition: One U =1 pmol of acetyl group removed/min/µg of enzyme
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Scher, M.B. et al., Genes Dev. 21 (8), 920-928 (2007)
2. Bellizzi, D. et al., Genomics 89 (1), 143-150 (2007)
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