Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and HTS applications.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The SMYD2 Chemiluminescent Assay Kit is designed to measure SMYD2activity for screening and profiling applications. The key to the SMYD2 Chemiluminescent Assay Kit is a highly specific antibody thatrecognizes a methylated residue of Histone H3. With this kit, only three simple steps are requiredfor methyltransferase detection. First, S-adenosylmethionine is incubated with a samplecontaining assay buffer and the SMYD2 methyltransferase enzyme. Next, primary antibody isadded. Finally, the plate is treated with an HRP-labeled secondary antibody followed by additionof the HRP substrate to produce chemiluminescence that can then be measured using achemiluminescence reader.
Storage Stability: One year from date of receipt when stored as directed.
Supplied As: The SMYD2 Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 substrate, antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Brown MA, et al. Mol. Cancer 2006; 5:26.