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Encompassing Amino Acids: 2-804(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Add 50 µl reaction mix (100 µM S-adenosylmethionine, SMYD4 enzyme, and HMT buffer 7Z' (BPS #52193Z) to wells coated with the substrate. Incubate 3-16 h. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
Description: Human SET and MYNDcontainingprotein 4 (SMYD4), alsoknown as KIAA1936, amino acids 2-804(end), with N-terminal FLAG-tagGenBank Accession No. NM_052928,MW = 90 kDa. This protein is expressedin a Baculovirus infected Sf9 cellexpression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 0.2 mM TCEP, 80 µg/mlFLAG peptide
Genbank: NM_052928
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: Q8IYR2
Warnings: Avoidfreeze/thaw cycles. Storing diluted enzymeis not recommended.
Biosafety Level: Not applicable (BSL-1)
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