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Application: Screen or titrate neutralizing antibodies against the SARS-CoV-2 Spike Omicron JN.1 variant in ACE2-expressing cells.
Background: The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As the first step of the viral replication, the virus attaches to the host cell surface before entering the cell. The viral Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer protection against the viral infection.Spike Mutations in JN.1 Omicron Variant:
T19I, LPP24-26del, A27S, S50L, HV69-70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, L455S, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, N764K, D796Y, S739F, Q954H, N969K, P1143L
Description: The Spike (JN.1 Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter) are replication incompetent, HIV-based lentiviral particles. They were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1 containing all the Omicron JN.1 mutations; see below for details) as the envelope glycoprotein, instead of the commonly used VSV-G. These pseudovirions also contain an eGFP reporter driven by a CMV promoter, allowing to measure the spike-mediated cell entry using the eGFP fluorescence signal.The pseudovirions have been validated in a cellular assay with ACE2-HEK293 Recombinant Cell Line (#79951), a cell line that overexpresses ACE2 at high levels, as target cell line.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: The titer will vary with each lot; the exact value is provided with each shipment. Based on experiments performed by scientists at BPS Bioscience, 78983-1 (100 µl size) provides sufficient pseudovirions to create a signal-to-noise ratio that allows to perform 100 reactions, and 78983-2 (500 µl x 2) for 1000 reactions. The amount of virus added to the cells can be titrated further down according to the user's need.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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