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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done according to the Spike RBD (B.1.1.7 Variant) (N501Y)
(SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit (BPS Bioscience #78140) with various amounts of ACE2, His-Avi-Tag, Biotinlabeled HiP™ (BPS Bioscience #100665).
Description: Recombinant SARS-CoV-2 Spike protein, RBD (Receptor Binding Domain) encompassing amino acids 319-541. This protein corresponds to SARS-CoV-2 Variant B.1.351, orignally discovered in South Africa and contains mutations K417N, E484K and N501Y. It also contains a C-terminal Avi-Tag™ and a C-terminal His-tag. The recombinant protein is ≥90% pure following high affinity Ni-NTA purification and shows less than 5% aggregation in gel filtration.
Formulation: 8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Genbank: MN908947
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal Avi-His-tag
Uniprot: P0DTD1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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