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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: SARS-CoV-2 S1(BPS Bioscience #100720) and ACE2 binding assay was done using BPS ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit (BPS Bioscience #79936). SARS-CoV-2 S1 (BPS Bioscience #100720) protein and Streptavidin-HRP were used instead of SARS-CoV-2 Spike protein (RBD) and anti-mouse Fc-HRP respectively.
Description: Severe acute respiratory Coronavirus 2 Spike Glycoprotein S1 (SARS-CoV-2 Spike S1), GenBank Accession No. QHD43416.1, a.a. 16-685, fused at the C-terminus of the Fc portion of human IgG1, with a C-terminal Avi-tag, expressed in a HEK293 expression system enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW=104 kDa. This protein runs at a higher molecular weight due to glycosylation.
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: QHD43416.1,
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal Fc-Avi-tag
Uniprot: P0DTC2 SPIKE_WCPV
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Hoffman, M. et al., Cell. 2020 Apr; 181:1-10.
2. Walls, A. C. et al., Cell. 2002 Mar; 180:1-12
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