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Encompassing Amino Acids: 1-368(full length)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: STK19 was diluted with kinase dilution buffer and 1 mg/ml CREBtide (KRREILSRRPYSYR) and assayed according to the ADP-Glo™ Kinase Activity Assay Kit (Promega, #V9101).
Description: Human Serine/threonine-protein kinase 19 (STK19), also known as G11 and RP1, GenBank Accession No. NM_004197, a.a. 1-368(full length), with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system. MW=70 kDa.
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 MM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_004197
Purity: >75%
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal GST-tag
Uniprot: P49844
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yin, C. et al., Cell. 176(5): 1113-1127, 2019.
2. Qian, L. et al., Clin. Cancer Res. 26(13): 3420-3430, 2020.
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