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Encompassing Amino Acids: 2-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using 5 µg/ml STK33 in 10 mM Tris, pH 7.4, 5 mM MgCl2, 0.5 mM EDTA, 0.5 mM DTT, 5 µM ATP, and 400 µg/ml MBP substrate for 1 hr. at room temperature.
Background: STK33 is a distant member of the CAMK group of serine/threonine kinases but it lacks the calcium/calmodulin-binding domain and the C-terminal regulatory tail. STK33 is highly expressed in testis, lung epithelia, alveolar macrophages, horizontal cells in the retina and in embryonic organs such as heart, brain and spinal cord. STK33 is essential for abnormal cell growth in human cell lines expressing oncogenic mutations in KRAS, but not in human cancer cell lines expressing wildtype KRAS. STK33 is required for survival and proliferation of mutant KRAS-dependent cancer cells, in which it suppresses the S6K1-BAD proapoptotic signaling pathway.
Description: STK33 (GenBank Accession No. NM_030906.2), a.a. 2-end, with N-terminal GST-tag, MW = 84 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 115 mM NaCl, 10% glycerol, and 2 mM DTT.
Genbank: NM_030906.2
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9BYT3
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Mujica, A.O., et al., 2001. Gene 280:175-181.
2. Brauksiepe, B., et al., 2008. Biochemistry 9:25-37.
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