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Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Background: STK4 (serine/threonine-protein kinase 4), also known as MST1 (Mammalian STE20-like protein kinase 1), belongs to a family of proteins that share similarity with budding yeast serine/threonine kinase Ste20 (sterile-20). It is a core component of the Hippo signaling pathway, the pathway that regulates organ size via cell proliferation or apoptosis and is widely expressed throughout the human body. In the presence of stressful stimuli, autophosphorylation of STK4 at Thr183 initiates caspase-mediated cleavage to create a highly active, 36 kDa STK4 fragment. Examples of stressors are okadaic acid (protein phosphatase PP-1 and PP-2A inhibitor) and staurosporine (a broad-spectrum protein kinase inhibitor). Overexpression of STK4 can initiate apoptosis in mammalian cells when transiently expressed with MST2, a closely related Class II GC (protein Ser/Thr) Kinase, or in the presence of SAPK (stress-activated protein kinase). Inhibition of STK4 can lead to failure to arrest proliferation or to developmental apoptosis in certain cell types. STK4 is a potential diagnostic biomarker and therapeutic target for the treatment of various types of cancer.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The STK4(MST1) Kinase Assay Kit is designed to measure STK4(MST1) kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant STK4(MST1) kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Uniprot: Q13043
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Praskova M, et al., Biochem J. 2004; 381(Pt 2):453-462.
Li W, et al., J Clin Invest. 2015 Nov 2; 125(11):4239-4254.
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