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Encompassing Amino Acids: 2-387
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl2, 4 mM DTT, 0.05% Tween-20, 800 µM S-adenosyl methionine, and SUV4-20H1) is added to the wells coated with the substrate. Incubate for 4 hr. Add primary antibody 18, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
Description: Human SUV4-20H1 (GenBank Accession No. NM_017635), a.a. 2-387, with N-terminal GST-tag, MW= 70 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_017635
Host Cell Line: E. coli
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q4FZB7
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yang H. et al., 2008 J Biol Chem. 2008 May 2;283(18):12085-92.
2. Benetti R et al., J Cell Biol. 2007 Sep 10;178(6):925-36.
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