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Encompassing Amino Acids: 2-739 (end)
Applications: Useful for binding studies to investigate protein-protein interactions in the EZH2 complex. Also suitable as an immunogen or as a standard for Western blotting.
Description: Human SUZ12 (suppressor of zeste 12), Genbank Accession #NM_015355, (a.a. 2-739 (end)) with N-terminal His and FLAG-tags, MW = 85 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.05% Tween-20, 20% glycerol, 1 mM DTT, and 100 µg/ml FLAG peptide
Genbank: NM_015355
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-FLAG-tags
Uniprot: Q15022
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Cao, R, Zhang, Y. Mol Cell. 15(1): 57-67 (2004).
2. Brecqueville, M, et al. Blood Cancer J. 1(8):e33 (2011).
3. Martin-Perez, D., et al. Am J Pathol. 2010 Aug;177(2):930-42
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