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Encompassing Amino Acids: 359-635(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: SYK was incubated with a substrate (Tyr peptide 2) for 1 hour at RT in 1X kinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Description: Recombinant human SYK, catalytic subunit (GenBank Accession # NM_003177), a.a. 359-635(end) with an N-terminal GST-tag, was expressed in Sf9 insect cells using a baculovirus expression system. MW = 58 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol and 3 mM DTT.
Genbank: NM_003177
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P43405
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Takano, T. et al. Antioxid Redox Signal. 2002 Jun,4(3):533-41.
2. Navara, C S. Curr Pharm Des. 2004,10(15):1739-44.