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Applications: Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.
Description: T4-phage beta-glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, Genbank Accession No. NP_049658, a.a. 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NP_049658
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P04547
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Song, C.X., et al. (2011). Nat Biotechnol. 29(1): 68-72.
2. Kornberg, S.R., et al. (1961). J. Biol. Chem. 236: 1487-1493.
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