Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
Description: The TAF1 (BD1+BD2) TR-FRET Assay Kit is designed to measure the inhibition of TAF1 (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing trFluor™ europium (Eu)-labeled TAF1 (BD1+BD2), dye-labeled acceptor, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Storage Stability: At least 6 months from date of receipt when stored as directed.
Supplied As: Kit comes in a convenient format with purified Europium-labeled TAF1 (BD1+BD2) protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.
Uniprot: P21675
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Filippakopoulos, P., et al., Cell 2012; 149:214.
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