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Encompassing Amino Acids: 849-1166(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted using BPS's TNKS2 HistoneRibosylation Assay Kit (Biotin-labeled NAD+),Catalog # 80578.
Description: TNKS2 (TRF1-interacting ankyrin-related ADP-ribose polymerase 2), also known as PARP5B, tanykyrase-2, or TNKL (GenBank Accession No. NM_025235), a.a. 849-1166(end), with N-terminal GST-tag, MW=62kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16mM glutathione, 20% glycerol.
Genbank: NM_025235
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-GST-tags
Uniprot: Q9H2K2
Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kaminker PG, et al. (2001). J. Biol. Chem. 276 (38): 35891-9.
2. Cook, B.D. et al. (2002). Mol. Cell. Biol. 22(1): 332-42.
3. Sidorova N, et al. (2006). Cell Tissue Res. 323 (1): 137-45.
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