Target: Tau.
Conjugate: HRP.
Product Type: Polyclonal.
Immunogen: Recombinant Human Tau 2NR4 P301S Fibril.
Swiss-Prot: P10636.
Purification: Protein A Purified.
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide.
Concentration: 1 mg/ml.
Specificity: Detects ~45.8kda.
Cellular Localization: Cytoskeleton, Plasma Membrane, Cell Membrane, Peripheral Membrane Protein, Cytoplasmic Side, Cytosol, Axon, Microtubule, Microtubule Associated Complex, Tubulin Complex, Nucleus, Nuclear Periphery, Nuclear Speck, Axolemma Plasma Membrane, Axolemma, Cytoplasm, Cell Body, Cytoplasmic Ribonucleoprotein Granule, Dendrite, Growth Cone, Neurofibrillary Tangle, Neuronal Cell Body.
Tissue Specificity: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
Scientific Background: Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). K18 is a truncated form of human tau containing only the 4 microtubule binding repeats (5). P301L (PL) is a mutation where proline is replaced by leucine at codon 301 of tau, and has been linked to frontotemporal dementia (6).
References: 1. www.alz.org/alzheimers-dementia/facts-figures2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907)3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497.4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18.5. Guo, J. and Lee, M.Y. (2013). FEBS Lett. 587(6): 717-723.6. Alberici, A. et al. (2004). J Neurol Neurosurg Psychiatry. 75:1607-1610.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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