Application Note: This antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 52 kDa in size corresponding to full-length TTFC. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 90 mg/mL
ELISA Dilution: 1:50,000 - 1:250,000
Western Blot Dilution: 1:1,000 - 1:40,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a polyhistidine-tagged TTFC fusion protein expressed in E. coli corresponding to full length TTFC.
Physical State: Liquid (sterile filtered)
Purity and Specificity: This antibody is directed against the C fragment of Tetanus toxin derived from Clostridium tetani. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest cross reactivity with Clostridium tetani. Cross-reactivity with TTFC from other sources have not been determined.
Background: Tetanus toxin C-fragment (TTc) is a non-toxic 52 kD polypeptide generated by papain cleavage of tetanus toxin, corresponding to the C-terminal 451 amino acids. The illness known as Tetanus is caused by a neurotoxin produced by the anaerobic bacterium Clostridium tetani. C. tetani is commonly found in soil, feces, and on many objects lying on the ground, such as rusty metal, hence puncture wounds are a common cause of tetanus. It acts upon the presynaptic membranes of both central and peripheral nervous systems to block the release of neurotransmitters. In its toxic form tetanus neurotoxin is a 150 kDa protein, consisting of two major components: a light chain and heavy chain. The light chain contains the enzymatic portion of the toxin and is responsible for its toxic effects. The heavy chain binds to the neuron and aids delivery of the light chain to the neuron interior. A single disulfide bond links light and heavy chains.
Low Endotoxin: No
Other: User Optimized
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