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Encompassing Amino Acids: 30-390 (end)
Application: Useful for cell culture and for the study of signaling and apoptotic pathways.
Biological Activity: The ED50 measured by TGFβ1's ability to inhibit mIL-4-dependent proliferation of HT-2 mouse helper T cells is <0.5ng/ml.
Description: Human TGFbeta1 (GenBank Accession No. NM_000660), a.a. 30-390 (end) with Cys33Ser mutation and N-terminal His-tag. Mature latent TGFbeta1 consists of disulfide-linked homodimers of TGFbeta1 latency-associated peptide (LAP) and mature TGFbeta1, MW= ~75 & 25 kDa under non-reducing conditions.
Endotoxin Level: < 1EU per µg
Format: lyophilized protein
Formulation: Lyophilized from a 0.2 µm filtered, aqueous solution
Genbank: NM_000660
Host Cell Line: FreeStyle 293-F cells
Reconstitution: Reconstitute in sterile 0.2 M acetic acid with 0.1% BSA. Further dilutions may be made in PBS with 0.1% BSA. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: >6 months at -80°C (as a solid). Store reconstituted protein in aliquots at -80°C for up to 3 months. Avoid freeze/thaw cycles.
Tags: none
Uniprot: P01137
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Derynck, R. et al. (1985). Nature 316(6030):701-5.
2. Bourdrel L., et al. (1993). Protein Expr. Purif. 4:130-140.
3. Munger, J.S., et al. (1997). Kidney Int. 51:1376-1382.
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