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Applications: Useful for studying protein binding and for screening small molecules and antibodies.
Assay Conditions: 100 nM of biotinylatedTIGIT was incubated for 1 hour with theindicated concentration of CD155 (Cat.#71181) in a 10 µl reaction in with TIGITAssay Buffer. Chelate Acceptor beads wereadded and incubated for 30 min. followed byStrep-donor beads for 30 min and alphacounts were read.
Description: Human T-cell immunoreceptorwith Ig and ITIM domains (TIGIT), alsoknown as V-set and immunoglobulindomain-containing protein 9 (VSIG9), andV-set and transmembrane domaincontainingprotein 3 (VSTM3), GenBankAccession No. NM_173799, a.a. 22-141fused to Fc region of human IgG1 andenzymatically biotinylated using AviTag™technology, expressed in a HEK293 cellexpression system. MW = 41.6 kDa(monomer). This protein runs at a higherMW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Genbank: NM_173799
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Fc fusion (IgG1), Avi-Tag™
Uniprot: Q495A1
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-57.
2. Stanietsky, N., et al., Proc. Natl. Acad. Sci. (USA). 2009; 106(42): 17858-17863.
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