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Encompassing Amino Acids: 22-200
Applications: Useful for studying protein binding and for screening small molecules and antibodies.
Description: Human TIM-3, also known as T-cellimmunoglobulin mucin receptor 3, T-cellmembrane protein 3, TIMD-3, Hepatitis Avirus cellular receptor 2, HAVCR-2, and KIM-3. GenBank Accession No. NM_032782,a.a. 22-200 with a C-terminal Avi-biotin, Histag,expressed in a HEK293 cell expressionsystem. MW = 23.3 kDa. This protein isenzymatically biotinylated using Avi-Tag™technology. Biotinylation is confirmed to begreater than 90%. This protein runs at ahigher MW by SDS-PAGE due toglycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Genbank: NM_032782
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Avi-biotin, His-tag
Uniprot: Q8TDQ0
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zhu, C. et al., Nat. Immunol. 2005;6:1245-1252.
2. Anderson, A.C. et al., Science. 2007;318(5853):1141-1143.