Application Note: Anti-TIP5 Antibody has been tested by ChIP and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 250 kDa in the appropriate cell lysate or extract.
Concentration Value: Neat
ChIP Dilution: 5 μl/ChIP
Western Blot Dilution: 1:1,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-TIP5 Antibody was produced in rabbits by repeated immunizations with a recombinant protein of human TIP5.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-TIP5 Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. Cross reactivity with TIP5 from other species not tested.
Background: TIP 5 is the large subunit of the nucleolar remodeling complex NoRC. NoRC causes the repression of ribosomal gene transcription. It was demonstrated that histone deacetylation is involved in this repression and that TIP5 is associated with the histone deacetylase HDAC1 and mediates the deacetylation of histones in the vicinity of the rDNA promoter. The interaction of TIP5 and HDAC1, which is necessary for transcriptional repression, is established by the C-terminal PHD finger and bromodomain. Anti-TIP5 Antibody is ideal for research in Gene Expression.
Low Endotoxin: No
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