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Encompassing Amino Acids: 388-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: TLK2 or Tousled-like kinase 2, is a nuclear serine/threonine kinases that is thought to be involved in the regulation of chromatin assembly (1). In addition, TLK2 is also involved in other cellular processes such as intracellular signaling cascade, protein amino acid phosphorylation, cell cycle, chromatin modification and in response to DNA damage stimulus. TLK2 displays maximal activity during S phase of the cell cycle where it appears to be regulated by cell-cycle-dependent phosphorylation. Inhibition of DNA replication causes a rapid loss of TLK2 activity, indicating that TLK2 function is tightly linked to ongoing DNA replication.
Description: Recombinant human TLK2 (a.a. 388-end), with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_006852
Host Cell Line: Sf9 cells
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q86UE8
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Yamakawa, A. et al: cDNA cloning and chromosomal mapping of genes encoding novel protein kinases termed PKU-alpha and PKU-beta, which have nuclear localization signal. Gene,1998; 202 (1-2): 193-201.
2.Sillje, H. H. W. et al: Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication. EMBO J. 18: 5691-5702, 1999.
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