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Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: Transmembrane protease, serine 2 is an enzyme that belongs to the serine protease family. It has been implicated as a target in prostate cancer. TMPRSS2 facilitates SARS-CoV-2 particle entry into host cells via S protein priming, and its inhibition blocks virus fusion with angiotensin-converting enzyme 2 (ACE2). This, in turn, restricts SARS-CoV-2 viral entry, making TMPRSS2 an important therapeutic target.
Description: The key to the TMPRSS2 Fluorescent Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for TMPRSS2 reactions. A sample containing TMPRSS2 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=383nm, λem=455nm) is measured using a plate reader. Camostat is supplied as a protease inhibitor control.
Storage Stability: One year from date of receipt when stored as directed.
Supplied As: The TMPRSS2 Fluorescent Assay Kit is provided in a convenient 96-well format, with purified TMPRSS2, TMPRSS2 Fluorogenic Substrate, and TMPRSS2 assay buffer for 96 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tomlins, S. A., et al.,2008. "Role of the TMPRSS2-ERG gene fusion in prostate cancer." Neoplasia 10(2): 177-IN9.2. Hoffmann, M., et al., 2020. "SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor". Cell 181: 271-280.
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