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Applications: This kit is useful for screening for inhibitors of TNF-alpha binding to TNFR2.
Background: Tumor necrosis factor receptor 2 (TNFR2, TNFRSF1B or CD120b) is a membrane receptor that binds the pleiotropic pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha). TNFR2 is predominately expressed in cells of the immune system, especially regulatory T cells, and by endothelial cells. TNFR2 exhibits neuroprotective properties and promotes tissue regeneration, making it a promising potential therapeutic target for the treatment of Alzheimer's disease, autoimmune diseases and cancer.
Description: The TNFR2:TNF-alpha[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of TNFR2:TNF-alpha. The key to this kit is the high sensitivity of detection of biotin-labeled TNF-alpha by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, TNFR2 is coated on a 96-well plate. Next, biotinylated TNF-alpha is incubated with TNFR2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Storage Stability: Up to 6 months from date of receipt, when stored as recommended.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled TNF-alpha, purified TNFR2, streptavidin-labeled HRP, and assay buffer for 96 binding reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Vanamee, ES., et al. Trends. Mol. Med. 2017, 23(11): 1037-1046Chen, X., et al. Sci. Signal. 2017, 10(462): eaal2328Ortí-Casañ. N., et al. Front. Neurosci. 2019, 13: 49
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