Shelf life (days): 365
Notes: Cayman's Total Collagen/Hydroxyproline Assay Kit provides colorimetric-based methods for measuring collagen/hydroxyproline in tissue, serum, and urine. In this assay, collagen is hydrolyzed to individual amino acids. Hydroxyproline, found almost exclusively in collagen, makes up 11.3% (Col I) -15% (Col III) by mass of the amino acid composition.6 Hydroxyproline is oxidized to a pyrrole intermediate, which reacts with Ehrlich's reagent to create a chromophore with peak absorbance at 560 nm. Both hydroxyproline and collagen standards are included. It is only necessary to use the standard that best fits the needs of your assay. In studies where hydroxyproline measurement is relevant (i.e., collagen metabolism or biosynthesis) the hydroxyproline standards may be used. The hydroxyproline standards may also be used to calculate collagen levels in appropriate samples by assuming hydroxyproline makes up 11-15% amino acid content of collagen by mass. For a direct measurement of collagen, the collagen standard may be used. Collagen standard will be hydrolyzed along with the samples to give hydroxyproline. The range of this assay is 0.02-10 µg for collagen and 0.006-3 µg for hydroxyproline with a lower limit of quantification (LLOQ) of 0.5 and 0.047 µg for collagen and hydroxyproline, respectively.
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