Products from BPS Bioscience require a minimum order value above 400€
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: TRAF6 activity was measured using varying amounts of TRAF6 and incubated with E1, E2, and ATP in the presence of an "intrachain Ubiquitin" mixture for 60 minutes at 30°C in a 20-µl reaction. After incubation, TR-FRET was detected (340=>620 nm, and 340=>665 nm). The TR-FRET is proportional to the auto polyubiquitination of TRAF6.
Description: Human Recombinant TRAF6 (TNF receptor associated factor 6), encompassing amino acids 51-159. This construct contains an N-terminal GST-tag followed by a PreScission Site to facilitate removal of the GST-tag.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, and variable Glutathione
Genbank: NM_145803
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-Tag
Uniprot: Q9Y4K3
Biosafety Level: Not applicable (BSL-1)
English
Deutsch
