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Encompassing Amino Acids: 456-822(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Activity determined using a DELFIA-assay with a poly-(Glu:Tyr 4:1)-biotin-coated plate, for 30 min. at room temperature.
Description: Human TRKB, also known as NTKR2 (Neurotrophic Tyrosine Kinase Receptor, type 2) or BDNF/NT-3 Receptor, GenBank Accession No. NM_001018064, a.a. 456-822(end), with N-terminal GST-tag, MW=67.5 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3 mM DTT, and 10% glycerol.
Genbank: NM_001018064
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q16620
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Naylor, R.L., et al. (2002). Biochem. Biophys. Res. Commun. 291 (3): 501-7.
2. Soppet, D., et al. (1991). Cell 65 (5): 895-903.
3. Squinto, S.P., et al. (1991). Cell 65 (5): 885-893.