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Encompassing Amino Acids: 456-825(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Activity determined using a DELFIA-assay with a poly-(Glu:Tyr 4:1)-biotin-coated plate, for 30 min. at room temperature.
Description: Human TRKC, also known as NTKR3 (Neurotrophic Tyrosine Kinase Receptor, type 3) or NT-3 Receptor, GenBank Accession No. NM_002530, a.a. 456-825(end), with N-terminal GST-tag, MW=68 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol
Genbank: NM_002530
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q16288
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lamballe, F., et al. (1991). Cell 66 (5): 967-979.
2. Tessarollo, L., et al. (1993). Development 118 (2): 463-75.
3. McGregor, L.M., et al. (1994). Genomics 22 (2): 267-72.