Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 29-159
Application: Useful for avidin pulldown and binding assays.
Assay Conditions: Assay was done according to TSLPR:TSLP [Biotinylated] Small Molecule Inhibitor Screening Chemiluminescence Assay Kit (BPS Bioscience
#82698) with various amounts of TSLP (R127A, R130A), Avi-Tag, His-Tag, Biotin-Labeled Recombinant.
Background: TSLP (thymic stromal lymphopoietin) is a protein that functions as a type I cytokine, as an alarmin and growth factor in the immune system. It is involved in type 2 immune responses, T
H2 (T helper 2 cells) responses, and the maturation and recruitment of dendritic cells (DCs), T cells, B cells, neutrophils, mast cells, and other lymphoid cells. It can be produced by epithelial and stromal cells in lung, skin, and gastric system, but also by DCs, basophils and mast cells. Its expression can be induced by infections, pro-inflammatory cytokines, proteases, and even mechanical injury. For instance, it can be produced in the lungs in response to infection with influenza or rhinovirus. Its role as alarmin can result in increasing inflammation. TSLP is linked to allergic reactions such as asthma, atopic dermatitis, and food allergies, by inducing the expression of OX40L, CD80 and CD86 and stimulating CD4
+ T cells. The mutations R127A and R130A function to remove a putative furin site and further stabilize the full-length protein in HEK293 cells. In 2021, the TLSP-neutralizing antibody tezepelumab was approved for the treatment of severe asthma. Targeting TSLP is an active area of investigation with ongoing clinical trials for the treatment of autoimmune disorders.
Concentration: 1.71 mg/ml
Description: Recombinant human TSLP (thymic stromal lymphopoietin), encompassing amino acids 29-159. This construct contains mutations R127A and R130A that impact the putative furin cleavage site present in the protein. This construct contains a C-terminal Avi-tag™ followed by a His-tag (6xHis). The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.
Endotoxin Level: 8.07 EU/mg
Format: Aqueous buffer solution.
Formulation: 8 mM phosphate, pH7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_033035.5
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: Avi-Tag, His-Tag
Uniprot: Q969D9
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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