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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: TSSK2 is an intronless serine-threonine kinase that was originally detected on mouse chromosome 16 (1). TSSK2 and TSSK1 share 72% overall amino acid identity and 83% identity in the kinase domain (2). Real-time PCR showed abundant TSSK2 expression in testis and lower levels in heart, brain, and placenta while Western blot analysis detected TSSK2 at the predicted molecular mass of 40 kDa in human sperm and testis. Co-immunoprecipitation and yeast 2-hybrid analysis show that TSSK2 can interact with a protein TSKS, that also acts as a substrate for this protein kinase.
Description: Recombinant human TSSK2, full length, with an N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system. The gene accession number is NM_053006.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_053006
Host Cell Line: Sf9 cells
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q96PF2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Nayak, S. et al: Immunohistochemical analysis of the expression of two serine-threonine kinases in the maturing mouse testis. Mech Dev. 1998 Jun;74(1-2):171-4.
2. Hao, Z. et al: Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues: a TSSK member is present in the equatorial segment of human sperm. Molec. Hum. Reprod. 10: 433-444, 2004.
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