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Encompassing Amino Acids: 1-479
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human TTBK1 protein, also known as Tau-tubulin kinase-1, GenBank Accession No. NM_032538, a.a. 1-479, with N-terminal GST-tag and C-terminal His-tag. MW = 81 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 2 mM glutathione
Genbank: NM_032538
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: N-terminal GST-tag, C-terminal His-Tag
Uniprot: Q5TCY1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sato, S., et al., Journal of Neurochemistry. 2006; 98(5): 1573-1584.2. Yu, N., et al., Neuroscience Letters. 2011; 491(1): 83-86.