Application Note: UBE2R1 Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. UBE2R1 Protein is suitable for use in Western Blot. Expect a band approximately ~33kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 0.1 µg/µL
Western Blot Dilution: User Optimized
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Recombinant full length human UBE2R1 was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >75% by densitometry.
Background: UBE2R1 is a member of the ubiquitin-conjugating enzyme family which catalyzes the covalent attachment of ubiquitin to other proteins. UBE2R1 is a part of the large multiprotein complex which is required for ubiquitin-mediated degradation of cell cycle G1 regulators and for the initiation of DNA replication (1). CK2 can phosphorylate CDC34 and UBC3B at corresponding serine residue in the C terminus of each protein. Phosphorylated UBC3B and CDC34 binds specifically to the F-box protein beta-TRCP which results in enhanced degradation of beta-catenin, a substrate of BTRC. CK2-dependent phosphorylation of CDC34 and UBC3B functions by regulating BTRC substrate recognition (2). UBE2R1 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cancer, Cell Cycle, and Neurobiology research.
Low Endotoxin: No
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