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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and UCHL3 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation 340 nm, emission = 460 nm.
Description: UCHL3, also known as Ubiquitin Carboxy-terminal Hydrolase L3 (GenBank Accession No. NM_006002), a.a. 2-231(end), with N-terminal His-tag, MW=27.1 kDa, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_006002
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P15374
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wada, H., et al. (1998). Biochem. Biophys.
Res. Commun. 251 (3): 688-92.
2. Nam, M.J., et al. (2004). Proteomics 3 (11):
2108-15.
3. Setsuie, R., et al. (2009). Neurochem Int.
54(5-6):314-21.
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