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Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and High Throughput (HTS) applications.
Background: ULK2 (also known as Unc51.2, unc-51 like autophagy activating kinase 2) is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy in response to nutrient starvation. Mammalian ATG13 binds ULK2 and mediates the interaction of the ULK protein with FIP200. The binding of ATG13 stabilizes and activates ULK and facilitates the phosphorylation of FIP200 by ULK. The ULK-ATG13-FIP200 complex is a direct target of mTOR and an important regulator of autophagy in response to mTOR signaling (1). Yeast 2-hybrid assays showed that the C-terminus of ULK2 binds the C-terminus of SynGAP, a negative regulator of Ras that is associated with neural development (2). Alterations in ULK signaling pathways may be involved in the formation of autophagy-regulated Lewi bodies, which have been associated with Parkinson's disease (3).
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The ULK2 Kinase Assay Kit is designed to measure ULK2 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The ULK2 Assay Kit comes in a convenient 96-well format, with enough purified ULK2, MBP substrate, ATP, and kinase assay buffer for 96 enzyme reactions.
Uniprot: Q8IYT8
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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