Storage: -20℃,12 months
Abbreviation: ALPL
Target Synonym: ALPL,AP-TNAP,APTNAP,HOPS,TNAP,TNSALP,alkaline phosphatase,liver/bone/kidney,TNALP
Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse ALPL concentrations in serum, plasma and other biological fluids.
Tested Sample Types: Serum, plasma and other biological fluids
Type: Sandwich-ELISA
Assay Time: 24h
Detection Type: Colorimetric method;ELISA;Sandwich
Detection Range: 0.78-50 ng/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ALPL . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ALPL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ALPL , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ALPL . You can calculate the concentration of ALPL in the samples by comparing the OD of the samples to the standard curve.
Cross_reactivity: This kit recognizes ALPL in samples.No significant cross-reactivity or interference between ALPL and analogues was observed.
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