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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP2 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured atexcitation = 340 nm, emission = 460 nm.
Description: Human USP2 (Ubiquitin-Specific Protease 2) or UPB41, GenBank Accession No. NM_004205, a.a. 259-605(end) with an N-terminal His-tag, MW=40.9 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 7.5, 110 mM NaCl, 2.2 mM KCl, 15 mM β-mercaptoethanol, 250 mM imidazole, and 20% glycerol
Genbank: NM_004205
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: O75604
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Stevenson, L.F., et al. (2010). EMBO J. 140(3): 384-96.2. Priolo, C., et al. (2006). Cancer Res. 66(17): 8625-323. Haimerl, F., et al. (2009). J. Biol. Chem. 284(1): 495-504.
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