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Encompassing Amino Acids: 798-1298
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: VEGFR3 was incubated with a substrate (Tyr peptide 4) for 1 hour at RT in kinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Description: Human VEGFR3 (GenBank Accession No. NM_182925), a.a. 798-1298 with N-terminal GST-tag, MW= 82 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.
Genbank: NM_182925
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P35916
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Goodyear S.M. et al., Prostate 69 (9), 982-990 (2009).
2. Ghalamkarpour A. et al., J. Med. Genet. 46 (6), 399-404 (2009).