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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 5 mM MOPS, 2.5 mM beta-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate VRK1 with 0.25 mg/ml dephosphorylated casein as substrate and [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Description: Human vaccinia related kinase 1 (VRK1), GenBank Accession No. NM_003384, full length with N-terminal GST tag, MW=71 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol, and 0.25 mM DTT.
Genbank: NM_003384
Host Cell Line: Sf9 cells
Storage Stability: Store at -80°C. Stable for >6 months when stored as directed.
Tags: N-terminal GST-tag
Uniprot: Q99986
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Nezu, J., et al., Genomics 1997;45:327-331.
2. Renbaum, P., et al., Am. J. Hum. Genet. 2009;85:281-289.
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