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Encompassing Amino Acids: 1-489
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 µl of varying amounts of WNK2 was mixed with 10 µl of 0.5 mg/ml PKA protein substrate. Reaction was initiated by adding 5 µl of 250 µM [33P]-ATP and incubating for 15 min at 30°C followed by spotting the reaction on phosphocellulose paper, fixing in 1% phosphoric acid, and assaying with a scintillation counter.
Background: WNK2 is a serine/threonine kinase of the WNK (with no lysine (K)) protein kinase subfamily that also include WNK1, 3 and 4. The WNK kinases feature a cysteine residue in place of a lysine residue conserved in subdomain II of most kinases. WNK2 is predominantly expressed in brain, heart muscle, small intestine, and colon. As a tumor suppressor, WNK2 is involved in the modulation of growth factor-induced cancer cell proliferation through the MEK1/ERK1/2 pathway. WNK2 expression was found down-regulated epigenetically by promoter methylation in human gliomas.
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_006648
Purity: 0,7
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal GST-tag
Uniprot: F8W9F9
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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