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Encompassing Amino Acids: 1-441
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 µl of varying amounts of WNK4 was mixed with 10 µl of 0.5 mg/ml PKA protein substrate. Reaction was initiated by adding 5 µl of 250 µM [33P]-ATP and incubating for 15 min at 30°C followed by spotting the reaction on phosphocellulose paper, fixing in 1% phosphoric acid, and assaying with a scintillation counter.
Background: WNK4 or WNK lysine deficient protein kinase 4 is a member of the WNK family of serine-threonine protein kinases which is part of the tight junction complex in kidney cells. WNK4 can regulate the balance between NaCl reabsorption and K(+) secretion. WNK4 negatively regulates the surface expression of SLC12A3, and the loss of this regulation may cause an inherited form of hypertension. WNK4 regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. WNK4 is a positive regulator for the WNK-OSR1/SPAK-NCC cascade and is a potential target of antihypertensive drugs.
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_175638
Purity: 0,85
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal GST-tag
Uniprot: Q80UE6
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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