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Encompassing Amino Acids: Full length
Applications: Western blot
Background: 14-3-3 epsilon, also known as tyrosine 3-mono-oxygenase/tryptophan 5-monooxygenase activation protein is a member of the 14-3-3 family. 14-3-3 epsilon has been reported to be involved in heat shock transcription factor 1 (HSF1) and extracellular signal regulated protein kinase (ERK) pathways (1). 14-3-3epsilon interaction with the deubiquitinating enzyme UBPY, also known as USP8, regulates cargo sorting and membrane traffic at early endosomes. Association of 14-3-3 epsilon, HSF1 with ERK during heat shock may modulate the amplitude of the response and control the termination of HSP expression on resumption of growth conditions (2).
Description: Mouse 14-3-3 epsilon Protein, GenBank Accession No. NM_009536, full length, with N-terminal His tag, expressed in Sf9 insect cells using baculovirus. MW = 33 kDa.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol
Genbank: NM_009536
Storage Stability: Store product at -80°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Tags: N-terminal His tag
Uniprot: P62258
Biosafety Level: Not applicable (BSL-1)
References: 1. Wang, X. et al: Interactions between extracellular signal-regulated protein kinase 1, 14-3-3epsilon, and heat shock factor 1 during stress. J. Biol. Chem. 2004; 279(47):49460-9. 2. Mizuno, E. et al: 14-3-3-dependent inhibition of the deubiquitinating activity of UBPY and its cancellation in the M phase. Exp Cell Res. 2007; 313(16):3624-34.
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