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Encompassing Amino Acids: Full length
Applications: Western blot
Background: 14-3-3 sigma or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents (1). The induction of 14-3-3 sigma is mediated by a p53 responsive element. Exogenous introduction of 14-3-3 sigma into cycling cells results in a G2 cell cycle arrest (2). Knockout of 14-3-3 sigma in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3sigma -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3sigma-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3 sigma plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death.
Description: Human 14-3-3 sigma Protein, GenBank Accession No. NM_006142, full length, with N-terminal His-tag, expressed in Sf9 insect cells using baculovirus. MW = 30 kDa.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol
Genbank: NM_006142
Storage Stability: Store product at -80°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Tags: N-terminal His tag
Uniprot: P31947
Biosafety Level: Not applicable (BSL-1)
References: 1. Chan, T A. et al: 14-3-3-sigma is required to prevent mitotic catastrophe after DNA damage. Nature 401: 616-620, 1999. 2. Hermeking, H. et al: 14-3-3-sigma is a p53-regulated inhibitor of G2/M progression. Molec. Cell 1: 3-11, 1997.
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