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Encompassing Amino Acids: Full length
Applications: Western blot
Background: 14-3-3 theta (also known as tyrosine 3-monooxygenase /tryptophan 5-monooxygenase activation protein, theta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. Through interaction with ASK1, c-jun NH-terminal kinase, and p38 mitogen-activated protein kinase (MAPK), 14-3-3 theta plays an important role in controlling apotopsis (1). Induced expression of 14-3-3 theta protein has been reported in patients with amyotrophic lateral sclerosis. Additionally, 14-3-3 theta has been observed to mediate nucleo-cytoplasmic shuttling of the N protein (coronavirus nucleocapsid protein) which causes severe acute respiratory syndrome (2).
Description: Human 14-3-3 theta Protein, GenBank Accession No. NM_006826, full length, with N-terminal His-tag, expressed in Sf9 insect cells using baculovirus. MW = 30 kDa.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol
Genbank: NM_006826
Storage Stability: Store product at -80°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Tags: N-terminal His tag
Uniprot: P27348
Biosafety Level: Not applicable (BSL-1)
References: 1. Lau, J.M. et al: The 14-3-3tau phosphoserine-binding protein is required for cardiomyocyte survival. Mol Cell Biol. 2007, 27(4):1455-66. 2. The severe acute respiratory syndrome coronavirus nucleocapsid protein is phosphorylated and localizes in the cytoplasm by 14-3-3-mediated translocation.J Virol. 2005 Sep;79(17):11476-86.
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