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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done according to the 3CL Protease, MBP-tagged (SARS-CoV-2) Assay Kit (BPS Bioscience #79955-1) with 3CL Protease (B.1.1.529, Omicron Variant) titrated from 90 ng/rxn to 0 ng/rxn.
Description: Purified recombinant SARS-CoV-2 protease 3CL (3C-like protease, also known as MPro) full length encompassing amino acids 1-306 (end). This construct does not contain a tag. The protein corresponds to SARS-CoV-2 variant B.1.1.529 (Omicron Variant), originally discovered in South Africa, and contains mutation P132H.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% Glycerol, and 3 mM DTT.
Genbank: YP_009725301
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)