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Encompassing Amino Acids: 1-306(full length)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Add 40 µl of 3CL protease diluted in 1x assay buffer. Start the reaction by adding 10 µl of diluted substrate. Immediately place plate in plate reader. Read kinetics for 60 min (λexc=360 nm, λem=460 nm). Total volume is 50 µl.
Description: Severe acute respiratory Coronavirus 3C-like protease (SARS-CoV 3CL Protease), GenBank Accession No. 1UK3_B, a.a. 1-306(full length), with a C-terminal His-tag, expressed in an E. coli expression system, MW=36 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 200 mM Imidazole
Genbank: 1UK3_B,
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal His-tag
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zhang, L., et al., Science. 2020 Apr; 368(6489): 409-412
2. Hsu, M.F. et al., JBC. 2005 Mar; 280:31257-31266.
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