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Application: Great for studying enzyme kinetics and HTS applications.
Background: Coronaviruses (CoVs) primarily cause multiple respiratory and intestinal infections in humans and animals. The 3CL protease, also known as Main Protease (Mpro), plays a vital role in processing the polyproteins that are translated from the viral RNA 3CL Protease inhibitors that can block viral replication are promising potential drug candidates that could be used to treat patients suffering with the COVID-19 coronavirus infection.
Description: The 3CL Protease Assay Kit is designed to measure 3CL Protease activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. 3CL inhibitor GC376 is also included as an inhibitor control.
Storage Stability: At least one year from date of receipt when stored as directed.
Supplied As: The kit comes in a convenient 96-well format, with purified 3CL Protease, fluorogenic substrate, and 3CL Protease assay buffer for 100 enzyme reactions.
Biosafety Level: Not applicable (BSL-1)
References: 1. Jared S. Morse, et al., 2020 Chem.Bio.Chem. 21:730 - 738.
2. Zhang, L., et al. 2020, Science 368 (6489): 409-412.
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