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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done according to the 3CL Protease (SARS-CoV-2) Assay Kit protocol (BPS Bioscience, #79955-1) with 3CL Protease titrated from 30 ng/rxn -0 ng/rxn.
Description: Severe acute respiratory Coronavirus 2 3C-like protease (SARS-CoV-2 3CL Protease), GenBank Accession No. YP_009725301, a.a. 1-306(full length), expressed in an E. coli expression system, MW=34 kDa.
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.
Genbank: YP_009725301
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zhang, L., et al., Science. 2020 Apr; 368(6489): 409-412
2. Hsu, M.F. et al., JBC. 2005 Mar; 280:31257-31266.
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