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Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay run according to 3CL Protease (T21I, S144A) (SARS-CoV-2) Assay Kit (BPS Bioscience #78834) with various amounts of 3CL Protease (T21I, S144A).
Description: Recombinant SARS-CoV-2 3C-like Protease, full length, encompassing amino acids 1-306(end). This construct does not contain a tag. The recombinant protein contains the mutations of interests (T21I) and (S144A) and is affinity purified.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Genbank: YP_009725301
Storage Stability: At least 6 months at -80°C.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)