Target: 4-Hydroxy-2-hexenal
Conjugate: ATTO 488
Product Type: Monoclonal
Clone Number: 5C11.1
Immunogen: Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)
Purification: Protein G Purified
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Scientific Background: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.
References: 1. Long, EK., Picklo, MJ Sr. (2010) Free Radic Biol Med. 49(1):1-8.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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